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Well ADAM is the basic building block of everything so you may need some to dsevelop your character further, I guess. Like you don't have to fight the Big Daddies in the first one, you can leave them be if you want. I'm guessing that's similar in this game. Although, who knows :3
You start off without a little sister. And you can adopt ones from dead big daddies but you have to protect them from other enemies. If you kill a few big daddies it is supposed that the Big Sister appears to attack. Although what if you then beat the Big Sister. It's the end of the game, that'd be pointless.
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